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基于系统发育和比较基因组分析揭示与发酵乳杆菌缓解结肠炎相关的关键基因 Article

赵岩, 张程程, 于雷雷, 田丰伟, 赵建新, 张灏, 陈卫, 翟齐啸

《工程（英文）》 2022年第17卷第10期页码 170-182 doi: 10.1016/j.eng.2020.09.016

摘要：

越来越多的研究表明，发酵乳杆菌可以用于溃疡性结肠炎的预防和治疗。本研究中，我们从中国不同地区的人群粪便样本中分离出了105 株发酵乳杆菌，并对其基因组草图进行了测序。我们挑选了3 株来自不同的系统发育支系的发酵乳杆菌（FWXBH115、FGDLZR121和FXJCJ61）和发酵乳杆菌模式菌株CECT5716，通过构建右旋糖酐硫酸钠（DSS）诱导的结肠炎小鼠模型，结肠炎相关的组织学指标，保护黏膜完整性，增加肠道短链脂肪酸（SCFA），显著减轻结肠炎，而其他两株菌未能提供类似的保护作用。

关键词：发酵乳杆菌结肠炎抗炎系统发育分析比较基因组分析

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肠道微生态——探索中药临床疗效的新思路 Review

陆艳蒙, 谢娇娇, 彭聪高, 王保红, 王恺岑, 李兰娟

《工程（英文）》 2019年第5卷第1期页码 40-49 doi: 10.1016/j.eng.2018.11.013

摘要：本文回顾了中药与慢性肝病、溃疡性结肠炎、肥胖和2型糖尿病等疾病之间的联系，从肠道微生态的角度阐明其潜在的机制。

关键词：中药肠道菌群脂肪肝溃疡性结肠炎肥胖糖尿病

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结直肠癌、基质和正常结肠黏膜显微解剖区域N-糖组的显著多样性 Article

Di Wang, Katarina Madunić , Tao Zhang, Guinevere S.M. Lageveen-Kammeijer, Manfred Wuhrer

《工程（英文）》 2023年第26卷第7期页码 32-43 doi: 10.1016/j.eng.2022.08.016

摘要：

Aberrant glycosylation is considered to be a hallmark of colorectal cancer (CRC), as demonstrated by various studies. While the N-glycosylation of cell lines and serum has been widely examined, the analysis of cancer-associated N-glycans from tissues has been hampered by the heterogeneity of tumors and the complexity of N-glycan structures. To overcome these obstacles, we present a study using laser capture microdissection that makes it possible to largely deconvolute distinct N-glycomic signatures originating from different regions of heterogeneous tissues including cancerous, stromal, and healthy mucosa cells. N-glycan alditols were analyzed by means of porous graphitized carbon liquid chromatography-electrospray ionization tandem mass spectrometry, enabling the differentiation and structural characterization of isomeric species. In total, 116 N-glycans were identified that showed profound differences in expression among cancer, stroma, and normal mucosa. In comparison with healthy mucosa, the cancer cells showed an increase in α2-6 sialylation and monoantennary N-glycans, as well as a decrease in bisected N-glycans. Moreover, specific sialylated and (sialyl-)LewisA/X antigen-carrying N-glycans were exclusively expressed in cancers. In comparison with cancer, the stroma showed lower levels of oligomannosidic and monoantennary N-glycans, LewisA/X epitopes, and sulfation, as well as increased expression of (core-)fucosylation and α2-3 sialylation. Our study reveals the distinct N-glycomic profiles of different cell types in CRC tumor and control tissues, proving the necessity of their separate analysis for the discovery of cancer-associated glycans.